Composite

Part:BBa_K4432321:Design

Designed by: Paul Weimer   Group: iGEM22_Evry_Paris-Saclay   (2022-09-26)


CrtO, idi and dsx expression operon under the control of the T5 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2114
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2114
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2288
    Illegal BamHI site found at 2295
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2114
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2114
    Illegal NgoMIV site found at 569
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3049


Design Notes

T5 promoter (BBa_K4432000) + custom made RBS (BBa_K4432011) + CrtO coding sequence (BBa_K4432021) + custom made RBS (BBa_K4432012) + idi coding sequence (BBa_K3166068) + custom made RBS (BBa_K4432013) + dxs coding sequence (BBa_K3166061)

Source

DNA synthesis, PCR amplification & Assembly

References